The 32P-postlabeling assay for DNA adducts - PubMed
32P-postlabeling analysis is an ultrasensitive method for the detection and quantitation of carcinogen-DNA adducts. It consists of four principal steps: (i) enzymatic digestion of DNA to nucleoside 3'-monophosphates; (ii) enrichment of the adduct fraction of the DNA digest; (iii) 5'-labeling of the ….
The 32P-postlabeling assay for DNA adducts | Nature Protocols
Nov 1, 2007 · 32 P-postlabeling analysis is an ultrasensitive method for the detection and quantitation of carcinogen–DNA adducts. It consists of four principal steps: (i) enzymatic digestion of DNA to ...
32P-postlabeling analysis of DNA adducts - PubMed
32P-Postlabeling analysis is an ultrasensitive method for the detection of DNA adducts, such as those formed directly by the covalent binding of carcinogens and mutagens to bases in DNA, as well as other DNA lesions resulting from modification of bases by endogenous or exogenous agents (e.g., oxidative damage).
³²P-postlabelling for the sensitive detection of DNA adducts
32P-postlabelling is a technique originally described by Kurt Randerath and colleagues for the sensitive detection of damage produced in DNA by reactive chemicals or genotoxins. The procedure essentially entails the enzymatic digestion of DNA to nucleoside 3'-monophosphates which are then radioactiv …
32P-Postlabeling DNA Damage Assays | SpringerLink
32P-Postlabeling analysis is a powerful technique for detecting, identifying, and quantifying DNA adducts induced by mutagens or carcinogens. The method involves enzymatic digestion of the DNA sample to nucleoside 3′-monophosphates, and partial...
The 32P-postlabeling assay for DNA adducts - Springer Nature
32 P-postlabeling analysis is an ultrasensitive method for the detection and quantitation of carcinogen–DNA adducts. It consists of four principal steps: (i) enzymatic digestion of DNA to nucleoside 3′-monophosphates; (ii) enrichment of the adduct. … more.
32P-Postlabeling Analysis of DNA Adducts | Springer Nature …
32 P-Postlabeling analysis is an ultra-sensitive method for the detection of DNA adducts, such as those formed directly by the covalent binding of carcinogens and mutagens to bases in DNA, and other DNA lesions resulting from modification of bases by
32P-Postlabeling for Detection of DNA Adducts | SpringerLink
The 32 P-postlabeling assay has emerged as the major tool for measuring DNA adducts because of its ultrasensitivity and applicability to theoretically any DNA-damaging agent, irrespective of its chemical nature, including unknowns.
32P-Postlabeling Analysis of DNA Adducts | SpringerLink
32P-Postlabeling analysis is an ultrasensitive method for the detection of DNA adducts, such as those formed directly by the covalent binding of carcinogens and mutagens to bases in DNA, as well as other DNA lesions resulting from...
32P-postlabeling DNA damage assays: PAGE, TLC, and HPLC
32P-Postlabeling analysis is a powerful technique for detecting, identifying, and quantifying DNA adducts induced by mutagens or carcinogens. The method involves enzymatic digestion of the DNA sample to nucleoside 3'-monophosphates, and partial purification of the adducted nucleotides followed by th ….